Determination of Achatina fulica Mucus Activity on Staphylococcus aureus

Resumo

OBJECTIVE: This study aimed to evaluate the in vitro antimicrobial activity of Achatina fulica mucus on S. aureus and determine the minimum inhibitory concentration (MIC) of purified mucus on S. aureus ATCC 25923 and bovine intramammary isolates. METHODS: Mucus was purified by high-performance liquid chromatography, and the active fraction underwent protein quantification and broth microdilution. RESULTS: The MIC for ATCC 25923 was 50 µg/mL; for the isolates, it ranged from 12.5 to 100 µg/mL. Two isolates (6.7%) showed MIC of 12.5 µg/mL, three (10%) 25 µg/mL, twenty-three (76.6%) 50 µg/mL, and two (6.7%) 100 µg/mL. MIC₅₀ and MIC₉₀ were 50 µg/mL and 100 µg/mL, respectively. CONCLUSION: The purified mucus demonstrated antimicrobial activity with bactericidal action. These findings highlight its biopharmaceutical potential for future veterinary therapy against S. aureus-induced intramammary infections.

KEYWORDS: Achatina Fulica; Antibiotic Therapy Antimicrobial Minimum Inhibitory Concentration Bovine Mastitis; Staphylococcus Aureus.